SCREENING OF PHYTOCHEMICALS AND INSECTICIDAL POTENTIALITY OF THE ULATKAMBOL, ABROMA AUGUSTA EXTRACTIVES
By: Mahfuja Majid and Farzana Pervin
Key Words: Abroma augusta, Insecticidal activity, Tribolium castaneum, Phytochemical activity.
Vol11-No1-p081-99, December 2024.
AbstractThe chloroform, methanol, ethyl acetate, and acetone extracts obtained from the fruit shell, leaves, root bark, root wood, seed, stem bark, and stem wood of Abroma augusta were evaluated against Tribolium castaneum adults using a residual film assay. The potency of the chloroform extracts, based on the observed mortality of adult beetles, ranked in descending order as follows: seed (257.2156 μg/cm²) > leaf (438.459 μg/cm²) > root wood (639.031 μg/cm²) > root bark (1234.363 μg/cm²) > stem wood (1272.258 μg/cm²) > fruit shell (1752.395 μg/cm²). For the methanol extracts, seed (407.568μg/cm2) > leaf (529.930μg/cm2) > stem wood (1104.856μg/cm2) > fruit shell (2314.99 μg/cm2) > root bark (3389.414 μg/cm2) >root wood (4017.242μg/cm2) extracts. For the ethyl acetate extract, seed (587.074 μg/cm2) > leaf (668.952 μg/cm2) > root wood (1128.608 μg/cm2) > stem wood (1258.099 μg/cm2) > root bark (1580.342 μg/cm2) > fruit shell (1853.586 μg/cm2) extracts. For the acetone extract, seed (579.534 μg/cm2) > fruit shell (611.689 μg/cm2) > root wood (672.290 μg/cm2) > stem wood (1552.904 μg/cm2) > leaf (1566.299 μg/cm2) > root bark extracts (1804.709 μg/cm2) 48 h of exposure. No changes in the results were observed due to prolonged exposure, except for a proportional increase in mortality. However, significant mortality was observed within just 30 minutes of exposure, highlighting the exceptional potential of these dose-mortality experiments.
The whole plant was found to contain several alkaloids and secondary metabolites, including steroids, triterpenes, flavonoids, megastigmanes, benzohydrofurans, and their glycosides, as well as phenylethanoid glycosides. The phytochemical composition of Abroma augusta was investigated using standard analytical methods, revealing the presence or absence of phytochemicals such as tannins, flavonoids, phenolic compounds, alkaloids, saponins, steroids, cardiac glycosides, phlobatinnins, and anthraquinones. The proximate composition quantified the percentages of tannins, flavonoids, phenolic compounds, alkaloids, and saponins.
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Mahfuja Majid and Farzana Pervin
SCREENING OF PHYTOCHEMICALS AND INSECTICIDAL POTENTIALITY OF THE ULATKAMBOL, ABROMA AUGUSTA EXTRACTIVES
Vol11-No1-p081-99, December 2024.
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